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Protective effect of electroacupuncture on the barrier function of intestinal injury in endotoxemia through HO-1/PINK1 pathway-mediated mitochondrial dynamics regulation

Cellular viability Optimization of Sp concentration and its cytotoxicity parameters were established by treating of 1.5 10 5 HeLa or 1.2 10 5 SHSY-5Y cells with serial concentrations of the Sp (100 M1 mM) for 15 min or conducting a time-course (5 min48 h) using 250 M Sp, followed by MTT assay as per standard protocol (Thermo Fisher Scientific)

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When PBMC were incubated in liraglutide, the GLP-1 receptor signaling was upregulated, followed by increased INF- production and downregulated programmed cell death protein 1 expression on T-cells [37]