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The Intranuclear Binding of Testosterone and 5-alpha-androstan-17-beta-ol-3-one by Rat Prostate.J Biol Chem.1968,25,5953-5960 48

For digestion, 40-50 L of the reaction mixture was combined with 10 L 0.2% (w/v) RapiGest SF (Waters, Milford, USA) prepared in 50 mM ammonium bicarbonate buffer (pH = 7.8) Subsequently, 3.3 L of 45 mM DTT in 100 mM ammonium bicarbonate buffer was added to the mixture and incubated at 37 C for 30 min