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Nucleofections were performed with a protocol optimized for MEFs using the Neon electroporation system (Invitrogen) (cells resuspended in Gibco 1xPBS, 1350 mV, 1 pulse, 30 ms) or Amaxa nucleofection system (Lonza) with proprietary nucleofection protocols optimized for HCT116 or MEFs, and cells resuspended in Solution I (5 mM KCl, 15 mM MgCl 2 , 50 mM Mannitol, 120 mM sodium phosphate solution pH 7.2)