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Autocrine Interleukin-10 mediates Glucagon-Like Peptide-1 Receptor-Induced spinal microglial -Endorphin expression

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Essential supplies: Peptide vial (lyophilized powder) Bacteriostatic water (multi-dose vial) Insulin syringes (1 mL, 100-unit, 29-31 gauge) for drawing doses Larger syringe with needle (3 mL syringe with 18-21 gauge needle) for reconstitution Alcohol swabs or 70% isopropyl alcohol pads Clean, flat work surface Recommended additions: Disposable gloves (nitrile preferred) Sharps container for used needles Pen and label for marking the vial with date and concentration Calculator or the peptide reconstitution calculator Two notes on syringes