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Costs associated with diabetes-related complications taken from annually published sources were updated to the most recently published values, with costs from non-annually updated sources inflated to 2022 GBP using the Health and Community Health Services Index published by the Personal Social Services Research Unit (PSSRU

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GSH/GSSG-Glo assay GSH/GSSG ratio of CuCl 2 -treated HepG2 and HepG2 ATP7B-KO cells was measured by GSH/GSSG-Glo Assay (Promega) according to manufactures instructions

Further, the increased liver glutamate in BSO mice may stimulate the glutamate cystine antiporter [46], leading to increased export of glutamate from cells in exchange for cystine from plasma