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Beyond bile acids, the classical pathway involves the translocation of Lipopolysaccharides (LPS) from Gram-negative bacteria across a compromised intestinal barrier

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As the authors point out, the findings of their study may indicate a production system effect rather than a diet effect

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NO also modestly reduces CYP51A1 mRNA expression, though the effect is less pronounced than the reduction at the protein level, further supporting a predominant role for post-translational control [43]