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P values are shown in figures for comparisons between groups as indicated, one-way ANOVA with Tukeys post hoc test ( dm )

It was not until the 1950s that Jean Sterne demonstrated the effectiveness of metformin in treating diabetes in human trials for the first time (Sterne, 1963)

Funding This work was carried out with the financial support of Medical University of Lodz

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These effects were compared to those of untreated controls that were also maintained on a high-fat diet