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Then, samples were filtered through Amicon filter devices, 3 kDa MWCO (Millipore), and filters were washed three times with H 2 O

This activation upregulates tyrosinase and related enzymes that control pigment synthesis[2]

found that mice fed on diets supplemented with high sugar and fat had elevated plasma TMAO levels, accompanied with increased TNF- levels and decreased IL-10 levels, which led to myocardial fibrosis (98)